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. Author manuscript; available in PMC: 2010 Mar 26.
Published in final edited form as: Methods Mol Biol. 2009;590:165–176. doi: 10.1007/978-1-60327-378-7_10

Fig. 10.3.

Fig. 10.3

COBRA validation of PgR promoter methylation in OHT (tamoxifen-resistant MCF-7 cells) but not in untreated MCF-7 cells. Positive control (genomic DNA methylated with SssI) showed 100% in the interrogated region. Negative control (blood DNA) should little to no restricted bands by BstUI (not methylated in this region). The parental MCF-7 cells harbored no methylation in this region whereas tamoxifen-resistant OHT cells showed signs of methylation in the PgR promoter, potentially signifying the onset of gene silencing.