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Classic competitive antagonism of NECA-mediated increases in intracellular calcium by XAC at adenosine A1 and A3 receptors. CHO A1 (A) and CHO A3 (B) cells were pre-equilibrated with 0 μM (●), 0.1 μM (○), 1 μM (■), or 10 μM (□) XAC before stimulation with the adenosine receptor agonist, NECA. Data represent the mean peak response, expressed as a percentage of the peak response mediated by 1 μM NECA ± S.E.M. of three experiments conducted in triplicate.
