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. 2010 Apr;77(4):678–686. doi: 10.1124/mol.109.063065

Fig. 3.

Fig. 3.

The influence of 500 μM Brilliant Black BN on agonist and antagonist potency at adenosine A3 receptor. A, the intracellular calcium mobilization mediated upon exposure of CHO A3 cells to increasing concentrations of NECA in the absence (●) and presence (○) of 500 μM Brilliant Black BN. B, inhibition of the calcium response stimulated in response to the indicated concentration of NECA after 30-min exposure of CHO A3 cells to increasing concentrations of MRS1220 (circles; 100 nM NECA), XAC (squares; 10 nM NECA), and XAC-X-BY630 (diamonds; 10 nM NECA) in the absence (closed symbols) and presence (open symbols) of 500 μM Brilliant Black BN. C, the change in the negative logarithm of antagonist equilibrium dissociation constants (Δ pKB) at the A3 receptor. Values represent the pKB (in the presence) minus the pKB (in the absence) of Brilliant Black BN as calculated from Table 2. Data represent the mean ± S.E.M. of three to nine experiments conducted in duplicate.