Fig. 6.
The effect of Brilliant Black BN (500 μM) added 1 min before NECA stimulation on the potency of XAC (1 μM) at the adenosine A1 and A3 receptor. The concentration response of adenosine A1 (A) and A3 (B) receptor-mediated intracellular calcium mobilization in the absence (circles) or presence (squares) of 1 μM XAC (pre-equilibrated for 30 min) after the addition of HBSS (closed symbols) or 500 μM Brilliant Black BN (open symbols) at 15 s and NECA at 75 s. Data are the peak response expressed as a percentage of the peak response mediated by 1 μM NECA. Data represent the mean ± S.E.M. of three experiments conducted in triplicate.