Fig. 10.

Effects of heme depletion or repletion on PB-mediated induction of hepatic CYP2B and CYP3A in C57BL/6J HRI (−/−; KO) hepatocytes and corresponding HRI (+/+; WT) controls. A, treatments were carried out exactly as detailed under Materials and Methods. Values are mean ± S.D. of three separate experiments. Statistically significant differences between two individual values are as shown by the * (p < 0.001). B, qRT-PCR analyses of total RNA from cultured hepatocytes treated as in A are shown at the top. Values are mean ± S.D. of three separate experiments. Statistically significant differences between the two individual values are as shown by the * (p < 0.001). A magnified graph of the CYP2B mRNA values in untreated hepatocytes (non–PB-treated) included at the top are shown at the bottom. C, pulse-chase and CYP2B immunoprecipitation analyses of untreated and PB-pretreated hepatocytes are shown at the bottom. The relative [³⁵S]Met/Cys incorporated into equivalent aliquots of CYP2B immunoprecipitates monitored by scintillation counting is shown on the left. Statistically significant differences were observed between the two mean ± S.D. values each marked with the same symbol at p < 0.001. The corresponding Typhoon 9400 analyses of corresponding CYP2B immunoprecipitate aliquots subjected to SDS-PAGE are shown on the right.