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. 2010 Apr;77(4):497–507. doi: 10.1124/mol.109.061929

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Fig. 1. — Coimmunoprecipitation and colocalization of Slo1_VEDEC and Slo1_QEERL in HEK293T cells. Lysates of HEK293T cells heterologously expressing Myc-tagged Slo1_VEDEC and HA-tagged Slo1_QEERL were immunoprecipitated with either mouse anti-Myc (A) or anti-HA antibodies (B). Normal mouse IgG served as a negative control. The immunoprecipitated Slo1 proteins were detected with anti-HA or anti-Myc as indicated. C, colocalization of HA-tagged Slo1_VEDEC and Myc-tagged Slo1_QEERL in HEK293T cells visualized by confocal microscopy. Slo1_VEDEC was detected with anti-HA (green, a and b), and Slo1_QEERL was detected with anti-Myc (red, c and d). Merged signals of Slo1_VEDEC and Slo1_QEERL are shown in e to h. Boxed regions in e and f are magnified in g and h, respectively. Colocalization in merged images appear as a yellow signal.