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Coexpression of Slo1VEDEC reduces the surface Slo1QEERL and Slo1 in a dose-dependent manner. HEK293T cells were transiently cotransfected with Myc-tagged Slo1QEERL and HA-tagged Slo1VEDEC. The amounts of plasmids used are shown (in micrograms). A, results from representative cell-surface biotinylation assays. B, the total and surface expressions of Slo1 were quantified by densitometry and plotted as mean ± S.E.M. of three experiments.
