Fig. 3.

Motif-swapped constructs of Slo1. A, schematic drawing of motif-swapped Slo1 constructs (not to scale). Top to bottom: Long-VEDEC, Long-QEERL, Short-QEERL, and Short-VEDEC. All of the constructs encode channels with an HA-tag at the NH₂ termini. White boxes represent the identical amino acid sequences in all Slo1 constructs. Gray boxes indicate the VEDEC-specific regions, and the black boxes indicate the QEERL-specific regions. The last five amino acids swapped are shown by letters. B, expression of motif-swapped Slo1 constructs after transient transfection of HEK293T cells determined by immunoblot analysis using the antibodies indicated.