Fig. 6.

The VEDEC motif is sufficient to prevent Slo1 from being expressed on the cell surface. HEK293T cells were transiently cotransfected with Myc-tagged Short-QEERL and HA-tagged Short-VEDEC. The amounts of plasmids used are indicated (in micrograms). A, results from representative cell-surface biotinylation assays as well as analyses of total expression of the HA and Myc tags as indicated. Note that total expression of each splice variant is closely related to the amount of each plasmid used in transfection. B, quantification (mean ± S.E.M.) of densitometric analyses of three repetitions of this experiment. Note the reduction of surface expression of Slo1 when even small amounts of Short-VEDEC are present.