Fig. 3.

Current and fluorescence responses from the ρ1 receptor labeled with A5m at the L166C site. Current and fluorescence responses from receptors activated by 60 μM GABA in the absence and presence of 20 μM 3α5βP or 3α5βPOH (A), 3α5βPS or 3α5αPS (B), or β-estradiol or 10 μM picrotoxinin (PTXnin) (C). The top traces show currents (I), and the bottom traces show the concomitant changes in fluorescence (F). The modulators are sorted according to their proposed classification into groups. All drugs inhibit the electrophysiological response, but only β-estradiol reduces the change in fluorescence (shown with dotted line). D, the summary of effects on electrophysiology and fluorescence responses. ∗, p < 0.05; ∗∗, p < 0.01; ∗∗∗, p < 0.001.