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. Author manuscript; available in PMC: 2010 Mar 26.
Published in final edited form as: Cell Physiol Biochem. 2008 Dec 9;22(5-6):601–610. doi: 10.1159/000185544

Fig. 6.

Fig. 6

DTT does not reverse the effects of oxidation on A653C hERG1 channels. (A) I-V relationships for A653C channels before and after oxidation with 2 mM tbHO2 and subsequent treatment with 20 mM DTT (n = 10). The holding potential was −90 mV. Only mutant channels conduct inward currents at a Vt negative to −80 mV. Currents were normalized to the peak of the outward current (1.18 ± 0.24 μA at −30 mV) under control conditions. (B) G-V relationships for A653C channels under control conditions (V1/2 = −60.8 ± 0.5 mV, k = 9.6 ± 0.4 mV, n = 10) and after oxidation with tbHO2 (V1/2 = −67.6 ± 0.6 mV, k = 10.5 ± 0.5 mV, n = 10). Subsequent application of DTT (V1/2 = −59.7 ± 0.5 mV, k = 11.6 ± 0.5 mV, n = 10) did not reverse the effects of tbHO2 on minimum G/Gmax.