. Author manuscript; available in PMC: 2010 Mar 26.

Published in final edited form as: Cell Physiol Biochem. 2008 Dec 9;22(5-6):601–610. doi: 10.1159/000185544

Table 1.

Biophysical parameters for WT and mutant hERG1 channels.

Ala653 substitution	V_1/2	ΔV_1/2	k	ΔG₀	ΔΔG₀	n	τ_slow deactivation	τ_fast deactivation	A_rel	n
	mV	mV	mV	kcal/mol	kcal/mol		ms	ms
(WT)	−29±0.7	n.a.	7.4±0.2	−2.4±0.1	n.a	11	296±19	32±2	0.87	11
Gly	−34±0.6^*	−5	9.1±0.3^*	−2.3±0.1	−0.09	17	1014±49^*	137±12^*	0.57	11
Met	−44±0.5^*	−15	8.4±0.2^*	−3.2±0.1^*	−0.8	19	328±15	17±1^*	0.93	10
Cys	−59±0.7^*	−29	8.5±0.2^*	−4.1±0.1^*	−1.76	23	696±66^*	107±14^*	0.78	14
Trp	−58±0.5^*	−29	8.3±0.3^†	−4.2±0.2^*	−1.86	8	1175±52^*	49±4^*	0.33	11
Phe	−60±1.2^*	−31	7.6±0.2	−4.7±0.2^*	−2.38	6	701±23^*	61±3^*	0.68	10
Ile	−66±0.7^*	−37	8.9±0.3^*	−4.5±0.2^*	−2.09	8	393±12^*	31±2	0.84	8
Leu	−68±0.5^*	−39	8.9±0.2^*	−4.5±0.1^*	−2.16	8	410±7^*	35±2	0.78	11
Val	−83±1.3^*	−54	8.2±0.2	−6.1±0.2^*	−3.7	14	787±23^*	78±7^*	0.56	10

V_1/2 and k were determined from fitting G-V relationships to a Boltzmann function; τ_Slow and τ_Fast: slow and fast deactivation time constants measured at −130 mV; A_rel: agnitude of the fast component of deactivation/magnitude I_tail, measured at −130 mV; n.a., not applicable.

P<0.01;

^†

P<0.05