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. Author manuscript; available in PMC: 2010 Mar 26.
Published in final edited form as: Biochemistry. 2009 Mar 17;48(10):2087–2098. doi: 10.1021/bi8016284

Figure 5.

Figure 5

Stopped-flow fluorescence scans with dCTP opposite dAP with a dP/T. (A) One syringe contained 400 nM DNA and 2 μM RB69 pol in 50 mM MOPS buffer (pH 7) at 24 °C. The other syringe contained varying dCTP concentrations (0, 15, 50, 400, and 1000 μM from top to bottom) in MOPS buffer. Both syringes contained 2 mM CaCl2. The fitting plot was obtained from the estimated parameters based on Scheme 2. The experimental scans are in color, and the fitting curves are in black. (B) The amplitude vs dCTP concentration fit a hyperbolic equation with a Kdaapp of 67 ± 12 μM.