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. Author manuscript; available in PMC: 2010 Mar 26.
Published in final edited form as: Biochemistry. 2009 Mar 17;48(10):2087–2098. doi: 10.1021/bi8016284

Table 1.

Oligonucleotides Employed in the Rapid Chemical-Quench, Pulse–Chase, Chemical-Quench, Equilibrium Fluorescence, and Stopped-Flow Experiments with RB69 pol

oligonucleotide sequence
13pC primera CCG ACC ACG GAA C
20tA templatea GGC TGG TGC CTT G A* G GGG AAd
14pT primerb GC GGA CTG CTT ACT
18tA templateb GCG CCT GAC GAA TGA ACT
13pC primerc GC GGA CTG CTT AC
18tG templatec GCG CCT GAC GAA TGG ACT-Cy3
a

Oligonucleotides (dP/T*) used in rapid chemical-quench, pulse–chase, chemical-quench, equilibrium fluorescence, and stopped-flow experiments for RB69 pol.

b

Oligonucleotides (dP/T+) used with Sequenase in trapping dTTP.

c

Oligonucleotides (dP/T) used for equilibrium fluorescence titrations to determine the binding affinity of dCTP for the Sq–dP/T complex.

d

A*, 2-aminopurine (dAP).