Table 2.

Components, Concentrations, and Percentages of Complexes before and after Mixing in Sequenase Trapping Experiments

	initial concentration (μM)	before mixing		5 s after mixing
		concentration (μM)	% of complex	concentration (μM)	% of complex
syringe A
Sequenase	2.4
dP/Ta	3
Sequenase–dP/Ta		2.3	97.1	0.3	14
Sequenase–dP/Ta–dTTP		0	0	2	99.8
syringe B
RB69 pol	2
dP/Tb	2
dTTP	2	0.4		0
RB69 pol–dP/Tb		0.2	10.6	1.8	90.3
RB69 pol–dP/Tb–dTTP		1.6	79.9	0.004	0.2

^adP/T⁺ is 14pT/18tA in Table 1.

^bdP/T* is 13pC/20tA in Table 1. 2-Aminopurine is the templating base.