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. Author manuscript; available in PMC: 2010 Oct 1.
Published in final edited form as: J Infect Dis. 2009 Oct 1;200(7):1152–1161. doi: 10.1086/605696

Figure 5.

Figure 5

SLO-mediated DC apoptosis involves caspase activation and GAS association. Percentages of hypodiploid cells were determined by propidium iodide staining in DC exposed to live wild-type or mutant GAS strains for 2 h followed by a 20 h chase-period (A). Means±SD of at least three independent donors are shown. P<0.01 for comparison with untreated DC (iDC). DC exposed to wild-type (slo+) or SLO-deficient (slo−) GAS strains for 2 h or 20 h were evaluated by 2-color plot of propidium iodide staining (B) or GAS-association (C), each vs a fluorescent marker of activated caspases (FLICA). Percentages of FLICA+ DC that displayed hypodiploid nuclei (B) or were also labeled with GAS-antiserum (C) were determined. Quadrant gates of one representative experiment of three are shown.