Table 1.
Effect of the substrates glucose, pyruvate, and lactate in raffinose–mKRB–egg yolk extender on fresh and frozen–thawed sperm
| Sperm characteristics | Control | –Glucose | –Pyruvate | –Lactate | Substrate-free | |
| Fresh sperm | Motility (%) | 78.2 ± 8.0 | 70.8 ± 2.6 | 67.3 ± 8.0 | 54.9 ± 6.1 | 44.8 ± 3.9a |
| VSL (μm/s) | 17.4 ± 1.2 | 16.5 ± 3.3 | 10.9 ± 1.4 | 15.4 ± 1.0 | 11.1 ± 1.9 | |
| VCL (μm/s) | 124.2 ± 3.1 | 115.1 ± 8.6 | 109.8 ± 1.9 | 108.3 ± 2.9 | 102.4 ± 4.1 | |
| ALD (μm) | 6.9 ± 0.1 | 6.5 ± 0.5 | 6.5 ± 0.2 | 6.5 ± 0.1 | 5.8 ± 0.3 | |
| BCF (Hz) | 22.2 ± 0.8 | 23.3 ± 1.8 | 25.4 ± 1.6 | 23.4 ± 1.0 | 29.0 ± 0.6 | |
| Acrosomal integrity (%) | 84.5 ± 2.9 | 76.2 ± 1.9 | 79.6 ± 4.9 | 77.1 ± 2.4 | 81.4 ± 4.4 | |
| Frozen–thawed sperm | Motility (%) | 21.5 ± 1.4 | 23.2 ± 0.9 | 19.8 ± 1.3 | 13.8 ± 2.4a | 13.7 ± 1.5a |
| VSL (μm/s) | 3.9 ± 0.4 | 3.2 ± 0.5 | 3.9 ± 0.4 | 3.9 ± 0.3 | 4.4 ± 0.8 | |
| VCL (μm/s) | 85.4 ± 8.7 | 71.8 ± 4.7 | 89.2 ± 13.2 | 77.8 ± 5.5 | 69.3 ± 7.4 | |
| ALD (μm) | 4.2 ± 0.5 | 3.3 ± 0.2 | 4.1 ± 0.5 | 3.8 ± 0.2 | 3.2 ± 0.5 | |
| BCF (Hz) | 32.2 ± 3.7 | 31.3 ± 1.6 | 36.2 ± 1.8 | 34.1 ± 0.9 | 31.7 ± 2.5 | |
| Acrosomal integrity (%) | 68.1 ± 4.0 | 71.3 ± 5.8 | 70.0 ± 2.0 | 64.1 ± 5.3 | 70.6 ± 2.3 |
ALD, amplitude of lateral head displacement; BCF, beat cross frequency; VCL, curvilinear velocity; VSL, straight-line velocity.
Data are presented as mean ± SEM (n = 3).
a
Value significantly (P < 0.05) different from control value.