Table 2.
Effect of lactate in raffinose–mKRB–egg yolk extender on fresh and frozen–thawed sperm
| Lactate concentration (mM) |
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| Sperm characteristics | 0 | 10.79 | 21.58 | 32.37 | 43.16 | |
| Fresh sperm | Motility (%) | 47.4 ± 4.9 | 46.3 ± 13.2 | 61.3 ± 2.1 | 67.5 ± 3.8a | 55.1 ± 3.7 |
| VSL (μm/s) | 12.5 ± 1.7 | 8.8 ± 1.0 | 16.5 ± 2.6 | 12.3 ± 1.2 | 9.8 ± 3.0 | |
| VCL (μm/s) | 98.3 ± 6.3 | 86.0 ± 2.7 | 94.6 ± 10.0 | 92.9 ± 3.1 | 95.9 ± 6.5 | |
| ALD (μm) | 5.3 ± 0.4 | 5.3 ± 0.3 | 4.8 ± 0.6 | 4.7 ± 0.3 | 5.1 ± 0.3 | |
| BCF (Hz) | 26.4 ± 1.4 | 24.7 ± 1.3 | 22.5 ± 0.8 | 24.5 ± 0.8 | 25.6 ± 1.3 | |
| Acrosomal integrity (%) | 75.9 ± 0.5 | 71.7 ± 4.0 | 73.6 ± 5.9 | 75.1 ± 0.5 | 71.8 ± 1.8 | |
| Frozen–thawed sperm | Motility (%) | 11.3 ± 2.2 | 17.5 ± 3.5 | 19.7 ± 2.8 | 22.3 ± 4.0a | 12.6 ± 4.3 |
| VSL (μm/s) | 3.5 ± 0.6 | 2.8 ± 0.3 | 3.6 ± 0.2 | 3.8 ± 0.1 | 4.9 ± 0.5 | |
| VCL (μm/s) | 78.6 ± 8.2 | 75.0 ± 3.5 | 78.5 ± 4.2 | 95.0 ± 14.1 | 126.9 ± 5.1a | |
| ALD (μm) | 3.4 ± 0.6 | 3.3 ± 0.1 | 3.5 ± 0.2 | 4.5 ± 0.6 | 5.5 ± 0.4a | |
| BCF (Hz) | 44.8 ± 1.5 | 36.7 ± 1.8 | 39.8 ± 0.8 | 36.5 ± 2.3 | 42.9 ± 2.1 | |
| Acrosomal integrity (%) | 69.3 ± 2.9 | 71.6 ± 0.4 | 69.0 ± 0.2 | 69.2 ± 0.2 | 61.5 ± 1.8 | |
ALD, amplitude of lateral head displacement; BCF, beat cross frequency; VCL, curvilinear velocity; VSL, straight-line velocity.
Data are presented as mean ± SEM (n = 3).
a
Value significantly (P < 0.05) different from control value.