Table 3.
Effect of ATP in raffinose–mKRB–egg yolk extender containing 32.37 mM lactate on fresh and frozen–thawed sperm
| ATP concentration (mM) |
||||||
| 0 | 0.92 | 1.85 | 3.70 | 5.55 | ||
| Fresh sperm | Motility (%) | 74.7 ± 1.8 | 72.8 ± 8.1 | 79.2 ± 3.3 | 73.8 ± 6.6 | 55.6 ± 4.2a |
| VSL (μm/s) | 9.1 ± 1.7 | 10.1 ± 0.5 | 14.7 ± 3.3 | 7.9 ± 0.6 | 8.4 ± 1.4 | |
| VCL (μm/s) | 110.2 ± 9.9 | 116.9 ± 8.5 | 123.6 ± 5.2 | 101.5 ± 8.8 | 104.9 ± 7.9 | |
| ALD (μm) | 6.0 ± 0.1 | 7.6 ± 0.6 | 6.3 ± 0.6 | 6.8 ± 1.0 | 5.7 ± 0.4 | |
| BCF (Hz) | 29.9 ± 2.8 | 34.0 ± 1.7 | 29.7 ± 2.7 | 32.9 ± 2.6 | 31.2 ± 1.5 | |
| Acrosomal integrity (%) | 75.5 ± 6.3 | 78.9 ± 6.5 | 83.2 ± 1.6 | 82.5 ± 7.2 | 77.1 ± 7.1 | |
| Frozen–thawed sperm | Motility (%) | 20.6 ± 0.3 | 24.6 ± 0.9 | 35.3 ± 1.3a | 26.9 ± 1.4 | 25.7 ± 7.2 |
| VSL (μm/s) | 6.0 ± 1.2 | 5.0 ± 0.2 | 4.1 ± 0.6 | 4.4 ± 0.6 | 3.0 ± 0.4 | |
| VCL (μm/s) | 99.0 ± 8.3 | 89.3 ± 4.5 | 84.5 ± 4.1 | 85.8 ± 2.7 | 80.9 ± 3.9 | |
| ALD (μm) | 5.2 ± 0.8 | 4.4 ± 0.4 | 4.3 ± 0.1 | 4.3 ± 0.1 | 3.4 ± 0.1 | |
| BCF (Hz) | 37.1 ± 2.0 | 34.4 ± 0.6 | 35.0 ± 1.6 | 34.6 ± 5.0 | 37.2 ± 2.2 | |
| Acrosomal integrity (%) | 67.7 ± 2.3 | 68.4 ± 8.8 | 70.6 ± 4.6 | 61.7 ± 3.5 | 66.5 ± 3.8 | |
ALD, amplitude of lateral head displacement; BCF, beat cross frequency; VCL, curvilinear velocity; VSL, straight-line velocity.
Data are presented as mean ± SEM (n = 3).
a
Value significantly (P < 0.05) different from control value.