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. 2010 Apr;333(1):161–173. doi: 10.1124/jpet.109.161711

Fig. 2.

Fig. 2.

Norleual inhibits HGF-dependent Met phosphorylation and Gab1 association and phosphorylation in vitro. a, HEK293 cells were treated for 10 min with HGF at the indicated concentrations. Lysates were IP with anti-Met (DO-24) and immunoblotted (IB) with anti-Met or anti-phosphotyrosine (06-427) b, HEK293 cells were treated for 10 min with HGF and/or norleual at the indicated concentrations. Lysates were IP with anti-Met (DO-24) and IB with anti-Met (DQ-13) or anti-phosphotyrosine (HAM 1676, lot JLB03). c, HEK293 lysates were IP with anti-Met (DO-24) and IB with anti-Gab1. d, HEK293 lysates were IP with anti-Gab1 and IB with anti-Gab1 or anti-phosphotyrosine (HAM 1676). e, relative amounts (normalized to percentage of total Met in IP) of quantified IP/IB band intensities for control (black bars), 110 pM HGF (checkered bars), 110 pM HGF plus 20 pM norleual (white bars), and 20 pM norleual (gray bars)-treated HEK293 cells for phospho-Met (n = 4), phospho-Gab1 (n = 4), and Met associated Gab1 (n = 3). Error bars are standard deviation, and p values were determined by one-way analysis of variance followed by Tukey-Kramer multiple comparison test.