Fig. 6.

Norleual inhibits angiogenesis and Gab1/c-Met association in human umbilical vein endothelial cells. a, norleual inhibits angiogenesis in the ex vivo mouse aortic ring assay. Mouse aortas were cut into 0.5-mm sections and imbedded in Matrigel. Rings were incubated in EGM-2 media with (n = 8) or without (n = 6) 10⁻¹⁰ M norleual for 4 days. Digital photos of the rings were taken on day 4. Scale bar, approximately 2 mm. b, quantification of aortic ring angiogenesis. Areas covered by angiogenic sprouts were quantified from day 4 ring photos for control- (white bar) and norleual (black bar)-treated rings. Data are expressed as mean ± S.E.M. A p value of 0.012 was obtained from a two-tailed t test. c, HUVECs were treated for 5 min with HGF and/or norleual at indicated concentrations. Lysates were IP with anti-Met and immunoblotted (IB) with anti-Met and Gab1.