Fig. 7.
Norleual inhibits proliferation/migration and Erk phosphorylation in B16-F10 cells. a, confluent B16-F10 cells were scratched and the denuded area was allowed to heal for 6 h with or without HGF (312 pM) and HGF/norleual (10−11 M). Images at time of scratch and 6 h are shown. b, distance of scratch closure was measured in a blinded manner (n = 6). The difference between HGF and HGF + norleual groups was significant at 4 and 6 h. Data are expressed as mean ± S.E.M., n = 6. ∗, p < 0.01, norleual-treated versus HGF-treated cells. c, Western blot analysis of phospho-Erk1/2 and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) in B16-F10 cells. Cell lysates that were analyzed for phospho-Erk and GAPDH content were cells that were stimulated with and without scratching in the presence of HGF (312 pM) and/or norleual (10−11 M) for 2 h.