Fig. 8.

Norleual inhibits B16-F10 melanoma lung colonization. B16-F10 murine melanoma cells (400,000), treated in suspension with norleual at 10⁻¹¹ M or PBS vehicle, were injected into the tail vein of C57BL/6 mice. Mice received daily intraperitoneal injections of norleual (50 μg/kg) or PBS vehicle. a, after 14 days, the lungs from norleual-treated mice exhibited an obvious reduction in melanoma colonies compared with untreated controls. b, after removal, lungs were homogenized and total melanin content was determined spectrophotometrically and used to quantify total pulmonary melanoma colonization in vehicle-treated and norleual-treated cells. Ungrafted age-matched control lungs exhibited a background absorbance at 410 nm. Data are expressed as mean ± S.E.M., n = 5. ∗, p < 0.05.