Fig. 6.

Lack of apoptosis in STS-26T cells after GGTI-2Z/lovastatin treatment. HA14-1-treated cells were used as a positive control. A, STS-26T cells were treated as indicated. Data represent means of triplicate samples and are representative of two independent experiments. B, STS-26T cells were treated as indicated. Attached and detached cells were pooled, and whole-cell lysates were separated and probed for cleaved caspase-3. C, STS-26T cells were treated for 48 h with the indicated concentrations of lovastatin and/or GGTI-2Z or for 30 min with HA14-1. Nuclei were stained with Hoechst 33342, and live-cell imaging was performed on a LSM-510 microscope at 40× magnification.