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. 2010 Mar 8;120(4):1285–1297. doi: 10.1172/JCI36551

Figure 6. TLR7 and TLR8 induce chemoresistance of lung tumor cells.

Figure 6

A549 (A, C, and E) or SK-MES cells (B, D, and F) were cultured in 6-well plates with or without loxoribine (added at days 0, 3, 6, and 9). Cells were then treated or not with cycloheximide, cisplatine, carboplatine, doxorubicine, or Navelbine at day 12 (A–D), or treated or not with cisplatine or carboplatine in association with Navelbine or doxorubicine (E and F). (A and B) The colony number (shown below) was determined after Crystal Violet coloration. (C–F) Cell viability was analyzed at day 15 by the surviving fraction, calculated as [no. colonies after chemotherapy treatment/(no. cells seeded at day 0 × PE)] × 100, where PE is plating efficiency (calculated as no. colonies/no. cells seeded at day 0). Data represent mean ± SD from 3 independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001 versus unstimulated, Student’s t test.