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. 2010 Mar 8;120(4):1285–1297. doi: 10.1172/JCI36551

Figure 8. Loxoribine-induced chemoresistance is dependent on the TLR7-MyD88 signaling pathway.

Figure 8

A549 cells were transfected or not with TLR7 siRNA, MyD88 siRNA, or scrambled siRNA. (A and B) At 3 and 5 days after transfection, the mRNA expression levels of TLR7 (A) and MyD88 (B) were determined by quantitative RT-PCR. The fold decrease (arbitrary units) was obtained by 2–ΔΔCT. (C) 2 days after transfection, the cells were stimulated with loxoribine or LPS for 48 hours. Cells were then treated or not with cisplatine for additional 36 hours, and cell viability was analyzed by annexin V staining and flow cytometry analysis. The percentages of dead cells were determined as annexin V–positive cells relative to cells treated with cisplatine only. Values shown are calculated as relative percentage of dead cells subtracted from 100%. Data represent mean ± SD from 3 independent experiments.