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. 2010 Mar 24;120(4):1355–1361. doi: 10.1172/JCI38322

Figure 2. The 3 Abs differ in their location of binding on the C. neoformans capsule.

Figure 2

(A–E) Three-dimensional reconstructions of confocal fluorescence images of mAbs binding H99. (A) mAb 18B7 conjugated to Alexa Fluor 488 (green) binding H99. (B) mAb 12A1 conjugated to Alexa Fluor 488 (green) binding H99. (C) mAb 13F1 conjugated to Alexa Fluor 488 (green) binding H99. (D) mAb 18B7 conjugated to Alexa Fluor 488 (green) and unlabeled mAb 12A1 detected with anti-IgM–TRITC (red) binding H99. (E) mAb 18B7 conjugated to Alexa Fluor 488 (green) and unlabeled mAb 13F1 detected with anti-IgM–TRITC (red) binding H99. In all images, the cell wall is stained with calcofluor white (blue). Scale bar: 5 μm (A–E). (F) Distance in microns between mAb binding and the cell wall (measured from the fluorescence profiles in ImageJ). ANOVA was used to test for differences in the distance to the cell wall.