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. 2010 Mar 8;120(4):1298–1309. doi: 10.1172/JCI39566

Figure 5. Repression of miR-31 expression significantly affects murine lung cancer clonal growth and tumorigenicity.

Figure 5

(A) Colony formation was suppressed in ED-1 and ED-2 murine lung cancer cells relative to controls by engineered knockdown of miR-31 through transient anti–miR-31 transfection. The stained colonies are displayed in the bottom row. (B) Repression of in vivo lung tumorigenicity of the indicated transfected ED-1 cells after FVB mouse tail vein injections. The bars represent the percentage of lung lesion numbers for anti–miR-31 transfection of ED-1 cells relative to mice injected via tail vein with anti–miR-ctrl transfected ED-1 cells. Forty mice in total were used, and each group had 10 mice, with results pooled from 2 independent experiments, as described in Methods. *P ≤ 0.05, **P < 0.01. Error bars indicate SD in A and SEM in B.