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. 2010 Mar 15;120(4):1111–1124. doi: 10.1172/JCI40269

Figure 3. Kinetics of apoptosis in combination therapy.

Figure 3

(A) OT-1 T cells were mixed with EL-4 cells that had been loaded either with specific SIINFEKL or a control SIYRYYG (SIY) peptide. Target cells were either pretreated with TAX overnight or were left untreated. Chromium release assay was performed in duplicate 1, 2, and 4 hours after start of the incubation. The 2h and 4h data were obtained from different reproducible experiments. (B) For the detection of early apoptosis, the effector OT-1 T cells were labeled with DDAO-SE and incubated with target EL-4 cells loaded either with specific or control peptides at a 20:1 ratio. After the indicated incubation time, cells were stained with Annexin V–FITC and 7-AAD. The proportion of Annexin V–positive cells was measured within the population of tumor cells by flow cytometry. Typical result of 1 of 3 performed experiments is shown.