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. 2010 Mar 24;120(4):1043–1055. doi: 10.1172/JCI41376

Figure 1. Effect of CR on mitochondrial oxidative damage in aged kidney.

Figure 1

Experiments were performed on young mice or on aged mice subjected to AL or 12 months of CR. (A and B) Serum cystatin C levels (A) and 24-hour urinary albumin excretion levels (B) at the end of the experimental period. (C) Azan-stained and 8-OHdG–immunostained kidney sections. Original magnification, ×200 (Azan, bottom); ×400 (Azan, top, and 8-OHdG). (D) Quantitative analyses of Azan and 8-OHdG staining of glomerular and tubulointerstitial lesions. (E) Urinary 8-OHdG excretion levels during the observation period. Data are mean ± SEM. *P < 0.05 vs. young, †P < 0.05 vs. CR at the same time point. (F–H) 8-OHdG content (F), relative proportion of D-17 deletions (as percentage of WT; G), and frequencies of point mutations in cytochrome b gene (H) in mtDNA isolated from the kidney. Data are mean ± SEM. **P < 0.05. Each group includes 7–9 mice. (I) Correlation between D-17 prevalence and serum cystatin C level.