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(A and B) mRNA (A) and protein (B) expression levels of Sirt1 in kidney samples. (C) NAD contents in kidney samples. (D) IP to detect acetylation of Foxo3 and interaction between Sirt1 and Foxo3 in kidney samples. Data are mean ± SEM. *P < 0.05. Each group includes 7–9 mice. (E) Correlation between Sirt1 mRNA expression level and serum cystatin C level. (F) Correlation between Sirt1 mRNA expression level and D-17 prevalence.
