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. 2010 Mar 24;120(4):1056–1068. doi: 10.1172/JCI41563

Figure 5. Sirt1 deficiency is associated with increased apoptosis and fibrosis in kidney subjected to ureteral obstruction.

Figure 5

(A) Representative pictures (original magnification, ×200) and quantification of TUNEL-positive apoptosis in the renal medulla of Sirt1+/+ mice and Sirt1+/– mice 3 days after UUO (*P < 0.05). (B) Immunoblot for expression of the apoptosis marker cleaved caspase-3 in the entire kidney of Sirt1+/+ and Sirt1+/– mice 3 days after UUO (n = 4, densitometry, *P < 0.01 versus Sirt1+/+ mice). (C) Representative pictures (original magnification, ×200) and quantification of Sirius red staining on kidney sections of Sirt1+/+ and Sirt1+/– mice 7 days after UUO (*P < 0.05 versus obstructed kidney of Sirt1+/+ mice). (D) Levels of Col1 protein expression in the entire kidney of Sirt1+/+ and Sirt1+/– mice 7 days after UUO were assessed by immunoblot (n = 5, densitometry, *P < 0.05 versus obstructed kidney of Sirt1+/+ mice).