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. 2010 Mar 24;120(4):1056–1068. doi: 10.1172/JCI41563

Figure 6. Sirt1 activation is associated with reduced apoptosis and fibrosis in kidney subjected to ureteral obstruction.

Figure 6

(A) Representative images (original magnification, ×200) and quantification of TUNEL-positive apoptosis in the renal medulla of vehicle-treated or Sirt1 activator SRT1720–treated (100 mg/kg/d) wild-type mice 3 days after UUO (*P < 0.05). (B) Immunoblot for cleaved caspase-3 expression in the entire kidney of vehicle-treated or SRT1720-treated wild-type mice 3 days after UUO (n = 4, densitometry, *P < 0.0001 versus obstructed kidney of vehicle-treated mice). (C) Representative images (original magnification, ×200) and quantification of Sirius red staining on kidney sections of vehicle-treated or SRT1720-treated mice 7 days after UUO (*P < 0.05 versus obstructed kidney of vehicle-treated mice). (D) Levels of Col1 protein expression in the entire kidney of vehicle-treated or SRT1720-treated wild-type mice 7 days after UUO were assessed by immunoblot (n = 5, densitometry, *P < 0.05 versus obstructed kidney of vehicle-treated mice). V, vehicle; SRT, SRT1720.