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. Author manuscript; available in PMC: 2010 Mar 26.
Published in final edited form as: Invest Ophthalmol Vis Sci. 2009 Mar 25;50(10):4611–4617. doi: 10.1167/iovs.08-2586

Figure 3.

Figure 3

Higher clonal growth by p63-positive cell clusters. When dispase-isolated human limbal sheets were cultured on plastic in SHEM for 48 hours (Fig. 2), the cells were collected from areas with (+) and without (−) p63+ clusters by a laser-assisted microdissection microscope (A) and rendered into single cells by trypsin/EDTA. After being seeded on 3T3 fibroblast feeder layers for 14 days, cells derived from p63+ cluster areas exhibited more vivid clonal growth revealed by rhodamine B staining (B) and a significantly higher CFE than those from the noncluster areas (C, **P < 0.01). Bars, 50 μm.