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. Author manuscript; available in PMC: 2010 Mar 26.
Published in final edited form as: Invest Ophthalmol Vis Sci. 2009 Mar 25;50(10):4611–4617. doi: 10.1167/iovs.08-2586

Figure 4.

Figure 4

Comparison of clonal growth potential between sheets and single cells. Dispase-isolated limbal epithelial sheets were cultivated Sh/Pl or Sg/Pl after trypsinization for 27 days. As expected, epithelial growth measured by digitizing phase-contrast micrographs and plotted as the percentage of confluence in Sg/Pl was significantly greater (A) and faster (B, P < 0.01) than that in Sh/Pl. Afterward, the same number of single cells obtained from the above two cultures was seeded on 3T3 fibroblast feeder layers. The resultant clonal growth by Sh/Pl was more vivid (C) and had a significantly higher CFE (D) than that by Sg/Pl (**P < 0.01).