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. 2010 Jan 20;19(8):1438–1452. doi: 10.1093/hmg/ddq018

Figure 2.

Figure 2.

Time course analysis of differentially expressed genes identified using the combined rank list for 24-month-old YAC128 and controls. We analyzed the top 10 genes from the established combined rank list. YAC128 and wild-type littermate controls were analyzed at 3, 6, 9, 12 and 24 months of age by qPCR. In addition to Wt1 and Ddit4l, we analyzed Protocadherin 20 (Pcdh20), Scm-like with four mbt domains 2 (Sfmbt2), aspartoacylase 3 (Acy3) and polymerase (RNA) II (DNA directed) polypeptide A (Polr2a) that were included in the top 10 genes of the combined rank list for 24-month-old mice. Pcdh20 was up-regulated in YAC128 compared with controls at 3 months (P = 0.0186), 9 months (P = 0.0173), 12 months (P = 0.0012) and 24 months (P = 0.035) (A). Sfmbt2 was up-regulated in YAC128 compared with controls at 12 months (P = 0.0012) and 24 months (P = 0.0012) (B). Acy3 was up-regulated at 12 months (P = 0.0025) and 24 months (P = 0.0012) (C). There was a tendency for increase of Acy3 transcript levels also at 9 months (P = 0.056). Polr2a transcript levels were increased in YAC128 at 12 months (P = 0.035) and 24 months (P = 0.0047) (D). YAC128 data were normalized to the calculated average for the wild-type controls for each individual target and time point. The bars show the mean ± SEM for each target. Statistical analysis was performed using Mann–Whitney two-tailed U-test; *P < 0.05; **P < 0.01; ***P < 0.001. White and grey bars indicate wild-type controls and YAC128, respectively.