Table 1.

QPCR analysis of Coch mRNA expression in the av mouse cochlea at P30

	AV-3J #1	AV-3J #2	AV-3J #3	NC #1	NC #2	NC #3
Avg Ct: COCH	19.97	19.80	19.10	20.17	19.73	18.90
Avg Ct: GAPDH	24.13	21.80	22.43	21.43	22.07	21.10
ΔCt: COCH	−4.17	−2.00	−3.33	−1.27	−2.33	−2.20
	AV-3J - NC
ΔΔCt: COCH	−1.23
Fold change: COCH	2.35

qPCR results measuring Coch mRNA expression in the USH1F model (av-3J mouse cochlea) compared with control mouse cochlea at 30 days. av-3J mouse cochleae were found to have a 2.35-fold increase in Coch mRNA compared with control mouse cochleae. ΔCt represents normalized gene expression level (normalized to its own internal control housekeeping gene GAPDH). A ΔΔCt value (an average value) was obtained by averaging and comparing ΔCt for AV-3J and control samples. A fold change equals 2^(−ΔΔCt). A higher Ct value represents lower gene expression.