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. 2010 Jan 27;19(8):1577–1592. doi: 10.1093/hmg/ddq030

Figure 7.

Figure 7.

Loss of Kif3a results in a Hedgehog-dependent increase in neural crest cell proliferation. (A–F) BrdU incorporation in e12.5 WT and Kif3a CKO embryos. (A and B) BrdU incorporation in the dorsal nasal capsule of e12.5 WT and Kif3a CKO embryos. (C and D) BrdU incorporation around the oral cavity of e12.5 WT and Kif3a CKO embryos. (E and F) BrdU incorporation in the ventral nasal capsule of e12.5 WT and Kif3a CKO embryos. (G) Quantification of BrdU incorporation in WT and Kif3a CKO e12.5 embryos, *P = 0.002. (H–K) Gli1 expression and adjacent BrdU incorporation in oral ectoderm (oe) and facial mesenchyme in e12.5 WT and Kif3a CKO embryos. (L–O) Gli1 expression and adjacent BrdU incorporation in lateral facial mesenchyme in WT and Kif3a CKO embryos. (P) Quantitative RT–PCR analysis of Gli1 expression in micro-dissected WT and Kif3a CKO cranial neural crest cells treated with Shh-N, *P ≤ 0.01. (Q) BrdU incorporation in WT and Kif3a CKO cranial neural crest cells treated with various concentrations of Shh-N,*P = 0.0028. Frontonasal prominence (f), nasal pit (*), primary palate (pp), oral cavity (oc), maxillary prominence (mx), oral ectoderm (oe) and tongue (t). White scale bar = 10 µm, black scale bar = 100 µm.