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. Author manuscript; available in PMC: 2010 Aug 1.
Published in final edited form as: Anal Chem. 2009 Aug 1;81(15):6558–6561. doi: 10.1021/ac901172a

Figure 2.

Figure 2

(a) Extracted ion current for a series of 50 nL plugs with increasing concentrations of leu-enkephalin dissolved in 50% methanol, 1% acetic acid in water. Plugs were segmented with a 3 mm gap of air and pumped at 200 nL/min from a 150 μm i.d. Teflon tube. Ion signal is for MS3 at 556→397→278, 323, 380 m/z. (b) Expanded view of extracted ion trace for 3 plugs of 100 nM leu-enkephalin from (a). Pictures to the left show the electrospray tip when sample is emerging (top) and when air is emerging (bottom) and corresponding signals.