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High-throughput plug analysis. Extracted ion current for a series of 12 plugs of 200 nM leu-enkephalin in 50% methanol and 1% acetic acid samples. Each plug was 13 nL volume, separated by a 3 mm air gap, and pumped into the emitter at 600 nL/min. Ion signal is for MS3 at 556→397→278, 323, 380 m/z.
