Skip to main content
. Author manuscript; available in PMC: 2010 Mar 26.
Published in final edited form as: Innate Immun. 2009 Aug 26;16(2):115–127. doi: 10.1177/1753425909339815

Fig. 3.

Fig. 3

C1q induced changes in DC differentiation are not due to selective DC death. Mononuclear cells were isolated from PB and cultured with or without 25 μg/ml C1q. Cells were collected on days 1–4 and analyzed for the co-expression of CD14 and annexin V. (A) Annexin V analysis revealed that monocyte-DCs cultured in the presence of C1q did not have higher percentage apoptosis of CD14− or CD14+ cells than those cultured in G4 alone on day 3. A typical experiment is shown illustrated by dot plots gated on DR+ cells (n = 3). (B) Microscopic observation of monocyte-DCs cultured in the presence of C1q (right panel) did not show increased number of apoptotic cells by Wright stain analysis. C1q-treated cells (right panel) were smaller in size and displayed monocyte-like morphology, lacking the typical extending membrane processes (arrow) characteristic of iDCs developing in the presence of GM-CSF + IL-4 alone (left panel).