LC-DAD analysis of N-(1,1-dimethyl-1-allyl)-tryptophan (2) at 210 nm. Trace A, synthetic 2; Trace B, natural 2 in the crude organic extract of S. arenicola CNS-205; Trace C, enzymatic 2 from the CymD-catalyzed reaction of DMAPP and tryptophan (1); and Trace D, the CymD boiled control of the enzymatic reaction in C.