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. Author manuscript; available in PMC: 2010 Aug 15.
Published in final edited form as: Anal Chem. 2009 Aug 15;81(16):6929–6935. doi: 10.1021/ac901008c

Figure 3.

Figure 3

(a) Schematic drawings of various conformational states of α-synuclein with a micelle bound to it in the Im state. Green and red spheres indicate the fluorescent dyes, Alexa 488 and 594, respectively. (b)–(c) Color-coded diagrams of apparent FRET efficiency, EFRET, as a function of SDS concentration, [SDS], and temperature, with the protein conformations, U, I, F, and Im, assigned to different domains. Black dots in (b) designate the 50% two-state transition midpoints between different states from temperature ramp experiments in Ref. 8. The dots are connected by lines to guide the eye. The temperature range in (c), 28 – 41°C, is half as broad as in (b), 21 – 47 °C.