Table 1.
Strains and plasmids
| Strain or plasmid | Description | Reference or source |
|---|---|---|
| S. cerevisiae | ||
| InvSc1 | MATa/MATa leu2/leu2 trp1-289/trp1-289 ura3-52/ura3-52 his3-Δ1/his3-Δ1 | Invitrogen |
| E. coli | ||
| SM10 λpir | thi thr leu tonA lacY supE recA::RP4-2Tc::Mu pir | [23] |
| S17-1 λpir | thi pro hsdR hsdM+ ΔrecA RP4-2::TcMu-Km::Tn7 pir | [23] |
| JM3000 cpdA::kan | cpdA null mutation | E. coli Genetic Stock Center |
| S. marcescens | ||
| CMS376 WT | PIC strain number 3611 | Presque Isle Cultures |
| CMS737 | fimC-4 | [18] |
| CMS641 | crp-1 fimC-2 | [18] |
| CMS524 | cyaA-2 (transposon mutation) | [18] |
| CMS772 | crr-1 (insertion mutation) | [18] |
| CMS786 | crp-23 (transposon mutation) | This study |
| CMS592B | pigB::Tnmariner | This study |
| CMS613 | crp-1 (insertion mutation) | [18] |
| CMS794 | crp-1 pigB::Tnmariner | This study |
| CMS1687 | crp-Δ4 (deletion mutation) | This study |
| CHASM | pigmented environmental isolate | This study |
| K904 | pigmented ocular clinical isolate | This study |
| Nima | ATCC#29632 | Pryce Haddix |
| CMS1812 | Nima crp-1 | This study |
| Plasmids | ||
| pMQ117 | oripSC101ts, aacC1, oriT, Plac-lacZa, URA3, CEN6/ARSH4 | [30] |
| pMQ118 | nptII, rpsL, oriT, oriR6K, URA3, CEN6/ARSH4 | [30] |
| pMQ124 | oriiColE1, PBAD-lacZa, oripRO1600, orip15a, oriT, URA3, CEN6/ARSH4 | [30] |
| pMQ131 | oripBBR1, aphA-3, oriT, URA3, CEN6/ARSH4 | [30] |
| pStvZ3 | oriR6K lacZ nptII promoter probe, oriT, URA3, CEN6/ARSH4 | This study |
| pMQ236 | pMQ118 with I-SceI site | [30] |
| pMQ240 | pMQ117 + I-SceI meganuclease, oriT | [30] |
| pRMQS157/pcyaA | pMQ131 + cyaA | [18] |
| pRMQS166/pcrp | pMQ131 with crp ORF | This study |
| pEJK2/pcrp-Δ4 | pRMQS166 with crp-Δ4 | This study |
| pEJK3/pcpdA | pMQ131 + cpdA | This study |
| pEJK4/pcpdA-1 | pMQ131 + cpdA-1 (S70F,G80S) | This study |
| pRMQS242 | pMQ124 + His8-crp | This study |
| pRMQS260 | pMQ236 + crp-Δ4 | This study |
| pRMQS268 | pStvZ3 + pigA internal fragment | This study |