Table 2.

Effect of glucose, cAMP and mutations upon prodigiosin production.

Straina	Mediumb	Prodigiosinc
WT	LB	0.14 ± 0.02
WT	LB + glucose (2% w/v)	<0.01
crp-23	LB	0.88 ± 0.18
crp-23	LB + glucose (2% w/v)	<0.01
WT	M63 + glucose (0.4% w/v)	0.47±0.11
WT	M63 + glucose (2% w/v)	0.16±0.03*
WT	M63 + glucose (4% w/v)	0.16±0.02*
crp-Δ4	M63 + glucose (0.4% w/v)	1.20±0.23
crp-Δ4	M63 + glucose (2% w/v)	0.23±0.06*
crp-Δ4	M63 + glucose (4% w/v)	0.17±0.07*
cyaA-2	LB	0.63 ± 0.03
crr-1	LB	0.48 ± 0.01
crp-Δ4	LB	0.92 ± 0.16
WT + pMQ131 (vector)	LB	0.13 ± 0.03
WT + pcyaA	LB	0.15 ± 0.02
cyaA-2 + pMQ131 (vector)	LB	0.46 ± 0.04
cyaA-2 + pcyaA	LB	0.11 ± 0.02
crp-23 + pMQ131 (vector)	LB	1.19 ± 0.22
crp-23 + pcrp	LB	<0.01
crp-23 + pcrp-Δ4	LB	1.03 ± 0.03
pigB::Tnmariner	LB	<0.01
crp-1 pigB::Tnmariner	LB	<0.01
Nima	LB	0.30 ± 0.03
Nima crp-1	LB	3.93 ± 0.59
Nima	LB + cAMP (10 mM)	0.05 ± 0.01
K904	LB	0.33 ± 0.06
K904	LB + cAMP (10 mM)	0.13 ± 0.01
CHASM	LB	0.06 ± 0.01
CHASM	LB + cAMP (10 mM)	0.02 ± 0.01

^aAll strains in CMS376 background unless noted. All experiments were performed in LB liquid medium.

^bExperiments with LB were done in liquid medium, M63 were with solid medium.

^cA₅₃₄/A₆₀₀, measured at 16-18 h; for LB experiments, a representative experiment is shown with the average of 4 independent biological replicates per data point ± one standard deviation. For M63 experiments, data is an average of 8 independent replicates from two separate experiments per strain ± one standard deviation.