Fig. 1.
Effect of ecto-domain of the rat Nogo-66 receptor (NgR) (27–310) fused to a rat IgG [NgR(310)ecto-Fc] and methylprednisolone (MP) on myelin-induced inhibition of neurite outgrowth in chick dorsal root ganglia (DRGs) in vitro. NgR, not MP, significantly rescues the inhibitory effect of myelin. (A) Dissociated embryonic day 13 chick DRG neurons were plated on phosphate-buffered saline (PBS) or myelin (400 ng/well) in the presence of NgR(310)ecto-Fc or MP. (B) Quantification of neurite outgrowth per cell (n = 3) expressed as a percentage of PBS control ± SEM (n = 3). Scale bar, 200 μm. *P < 0.05 compared with PBS control.
