Fig. 5.
Effect of ecto-domain of the rat Nogo-66 receptor (27–310) fused to a rat IgG [NgR(310)ecto-Fc] and methylprednisolone (MP) treatment on the number of biotin dextran amine (BDA)-labeled axons contacting motor neurons in the ventral horn. BDA-labeled fibers represent corticospinal tract (CST) axons projecting from above the site of spinal cord transection. BDA-labeled axons projected from the ventral CST towards and into the gray matter of the ventral horn (A); vGLUT1-positive cells represent motor neurons in the ventral horn of the low thoracic/high lumbar region of the spinal cord (15 mm below the site of the lesion). BDA-labeled axons (red) abutted vGLUT1-positive cells (green) shown here by double staining (yellow) (B). The number of axons contacting motor neurons in the ventral horn was significantly increased in the MP + NgR(310)ecto-Fc-treated group compared with controls (C). *P < 0.05, one-way ANOVA with Dunnett’s posthoc test.
