Table 2.
Analysis of the transcripts identified by DDRT-PCR as upregulated in the LV/RV of newborn piglets.
| Gene identification | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Band number | Enriched in | Size, Bp | Primer Pair | Fold change* | Gene symbol | GenBank Acc. Nº | Base pair match | Species | Function |
| D005 | LV | 250 | H01-A05 | 2.1 ± 0.3 | TTN | AJ560658 | 98% | S. Scrofa | myofibrillar stretch-sensor system, |
| D015 | LV | 500 | H03-A04 | 1.7 ± 0.2 | MAP3K7IP2 | NM_015093 | 94% | H. sapiens | proliferation and anti-apoptotic signalling |
| D024 | LV | 275 | H04-A01 | 1.5 ± 0.2 | TNNT2 | AY277394 | 71% | H. sapiens | regulation of muscle contraction |
| D034 | LV | 450 | H06-A03 | 2.1 ± 0.3 | ATP5C1 | NM_005174 | 86% | H. sapiens | ATP synthesis |
| D036 | LV | 400 | H06-A04 | 4.2 ± 0.3 | ANKRD1-i8 | FJ475066 | 100% | S. scrofa | ANKRD1 splice isoform |
| D046 | LV | 400 | H08-A01 | 2.0 ± 0.3 | ADAMTS3 | BC132735 | 88% | H. sapiens | extracellular matrix degrading enzyme |
| D106 | LV | 450 | H08-A07 | 2.2 ± 0.4 | Unknown | — | — | — | — |
| D123 | LV | 1400 | H06-A10 | 4.2 ± 0.2 | PTPLAD1 | NM_001103316 | 90% | B. taurus | modulation of gene expression |
| D128A | LV | 900 | H05-A14 | 2.3 ± 0.2 | SLC8A1 | NM_001112802 | 87% | H. sapiens | calcium level control |
| D128B | LV | 900 | H05-A14 | 3.2 ± 0.5 | CTSH | EU532429 | 79% | S. scrofa | degradation of proteins in lysosomes |
| D130 | LV | 800 | H06-A14 | 4.2 ± 0.4 | PDE3A | XM_520783 | 77% | P. troglodytes | hydrolysis of cyclic nucleotides |
| D132 | LV | 310 | H08-A15 | 5.6 ± 0.4 | TPM2 | NG_011620 | 80% | H. sapiens | regulation of muscle contraction |
| D133 | LV | 350 | H10-A13 | 2.1 ± 0.4 | SERPINB9 | NM_004155 | 73% | H. sapiens | inactivation of serine proteinases |
| D134 | LV | 200 | H08-A18 | 2.6 ± 0.4 | ND6 | AY063320 | 96% | H. sapiens | ATP production |
| D137 | LV | 415 | H04-A18 | 2.6 ± 0.4 | MSTN | AY208121 | 95% | S. scrofa | negative regulator of muscle growth |
| D144 | LV | 180 | H03-A18 | 4.0 ± 0.2 | ACTC1 | FM212567 | 83% | S. scrofa | contractile apparatus assembling |
| D147 | LV | 500 | H02-A07 | 3.7 ± 0.3 | INHBA | NM_214028 | 86% | S. scrofa | myocardial remodelling and tissue repair |
| D151A | LV | 435 | H05-A07 | 2.1 ± 0.3 | Unknown | — | — | — | |
| D151B | LV | 435 | H05-A07 | 1.6 ± 0.2 | COL1A2 | NG_007405 | 77% | H. sapiens | formation of fibrillar collagen |
| D153 | LV | 300 | H05-A02 | 1.6 ± 0.3 | PHB2 | NM_001046198 | 80% | B. taurus | transcriptional repression regulation |
| D155 | LV | 930 | H10-A23 | 9.2 ± 0.3 | ANKRD1 | NM_213922 | 100% | S. scrofa | myofibrillar stretch-sensor system |
| D162 | LV | 930 | H05-A23 | 4.1 ± 0.5 | Unknown | — | — | — | — |
| D165 | LV | 1000 | H03-A24 | 2.1 ± 0.4 | Unknown | — | — | — | — |
| D170 | LV | 360 | H06-A21 | 10.8 ± 0.7 | NPPB | NM_213846 | 100% | S. scrofa | new NPPB putative splice isoform** |
| D006 | RV | 600 | H01-A05 | 2.5 ± 0.4 | Unknown | — | — | — | — |
| D42B | RV | 600 | H07-A01 | 3.1 ± 0.2 | CEP290 | NM_025114 | 89% | H. sapiens | transcription activation (via ATF-4 factor) |
| D050 | RV | 450 | H08-A04 | 2.0 ± 0.5 | SPTBN1 | AB371586 | 92% | H. sapiens | determination of cell shape |
| D051 | RV | 400 | H08-A05 | 1.9 ± 0.2 | SLC41A1 | NM_173854 | 78% | H. sapiens | magnesium transporter |
| D103 | RV | 350 | H06-A12 | 5.5 ± 0.2 | TNMD | XM_001088536 | 69% | M. mulatta | angiogenesis inhibitor |
| D104 | RV | 900 | H06-A07 | 2.1 ± 0.3 | APOBEC3F | FJ042939 | 83% | S. scrofa | growth and cell cycle control |
| D145 | RV | 375 | H08-A19 | 3.3 ± 0.4 | Unknown | — | — | — | — |
| D146 | RV | 850 | H05-A13 | 1.9 ± 0.2 | JMJD1C | NM_032776 | 91% | H. sapiens | hormone-dependent transcriptional activation |
*Fold change determined by qPCR. **The D170 band sequence exhibits homology with exon 1 and 3 sequences of the pig nppb gene.
ACTC1: actin, alpha, cardiac muscle 1; ADAMTS3: ADAM metallopeptidase with thrombospondin type 1 motif 3; ANKRD1: ankyrin repeat domain 1 (cardiac muscle); ANKRD1-i8: ANKRD1 retaining intron 8; APOBEC3F: apolipoprotein B mRNA editing enzyme, catalytic polypeptide-like 3F; ATP5C1: ATP synthase, H+ transporting, mitochondrial F1 complex, gamma polypeptide 1; CEP290: centrosomal protein 290 kDa; COL1A2: collagen, type I, alpha 2; CTSH: cathepsin H; INHBA: inhibin, beta A; JMJD1C: jumonji domain containing 1C; MAP3K7IP2: mitogen-activated protein kinase kinase kinase 7 interacting protein 2; MSTN: myostatin; ND6: mitochondrially encoded NADH dehydrogenase 6; NPPB: natriuretic peptide precursor B; PDE3A: phosphodiesterase 3A, cGMP-inhibited; PHB2: prohibitin 2; PTPLAD1: protein tyrosine phosphatase-like A domain containing 1; SERPINB9: serpin peptidase inhibitor, clade B (ovalbumin), member 9; SLC41A1: solute carrier family 41, member 1; SLC8A1: solute carrier family 8 (sodium/calcium exchanger), member 1; SPTBN1: spectrin, beta, non-erythrocytic 1; TNMD: tenomodulin; TNNT2: troponin T type 2 (cardiac); TPM2: tropomyosin 2 (beta); TTN: titin.