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. Author manuscript; available in PMC: 2010 Mar 28.
Published in final edited form as: J Immunol. 2007 Aug 1;179(3):1524–1531. doi: 10.4049/jimmunol.179.3.1524

FIGURE 1.

FIGURE 1

DT-induced depletion of DC in DTR chimeric mice. DTR→NT and NT→NT chimeras were treated with DT (4 ng/g body weight) or PBS on days −4 and −1, and on day 0 spleens were harvested. One half of each spleen was processed by mechanical disruption followed by digestion with collagenase D (1 mg/ml) for 1 h, whereas the other half was processed by mechanical disruption only. FACS plots show GFP vs CD11c expression on MHC class II+ gated cells, with the percentage of CD11c+GFP+ (DTR/GFP transgenic) and CD11c+GFP− (nontransgenic host-derived) DC indicated.