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. Author manuscript; available in PMC: 2010 Mar 28.
Published in final edited form as: J Immunol. 2007 Aug 1;179(3):1524–1531. doi: 10.4049/jimmunol.179.3.1524

FIGURE 5.

FIGURE 5

CD4+CD25+ Tregs are not required for CD4 cell tolerization to parenchymal self-Ag. Naive Thy1.1+ CFSE-labeled clonotypic CD4 cells were adoptively transferred into NT recipients infected with 106 PFU of viral-HA (viral-HA), C3-HAlow (C3-HA), and C3-HAlow recipients infected with 106 PFU of viral-HA (viral-HA + C3-HA) that had been treated 4 days earlier with either anti-CD25 (PC61) or control rat Ig, and were recovered from spleens for analysis 5 days posttransfer. A, Representative plots showing the frequency of CD4+CD25+ cells in peripheral blood both before and 4 days after Ab treatment. CD25 staining was performed using the 7D4 mAb, which does not cross-react with PC61. Representative CFSE-dilution histograms (B) and quantification of clonal expansion (C) and total TNF-α expression (D) are presented as in previous figures. n = 3 for each group.